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Post-doc or PhD projects available
[...] Neurodegeneration, Mitochondria-related disease, Alzheimers disease and related tauopathies, neurogenetic disease, and basic cellular neurobiology to join the laboratory Functional Genetics of Neurodegeneration and Neurologic Disorders under the lead of Hans Zempel, MD PhD MSc. The group currently consists of 1
technician, 1 post-doc, 4 PhD students, and 1-2 undergraduate students, and is embedded in the Institute of Human Genetics (University Hospital Cologne) , which has roughly 50 scientists and medical doctors, with joint progress reports and journal clubs together with collaborating neuroscience groups from adjacent research institutions. Your Profile PhD/ MSc in Biology, Biochemistry, Cellular Neuroscience or related disciplines High level of intrinsic motivation and reliability, open-minded to technology, willing to take on responsibilities and develop into a full-fledged scientist Collaborative work attitude and flexibility, scientific drive, courage to drive projects forward in spite of difficulties Experience in microscopy, cell culture work, and diverse biotech experience including animal work is highly favored Good oral and written communication skills in English; B2 [...] and test the effect of pharmacologic and genetic stimulation of MB in human primary fibroblasts and iPSC-derived neurons mimicking human disease. We have obtained several fibroblast lines from POLG-SD patients, have initially characterized them, and have shown that these cells
exhibit mtDNA reduction and loss of mitochondrial mass. We aim to establish a patient-derived cell study by collecting patient skin samples (used to generate fibroblasts) , characterizing them for mtDNA amount, mitochondrial mass, distribution, and function, as well as general cell parameters, and reprogram selected fibroblasts to iPSCs for targeted differentiation. Furthermore, we will create iPSCs mimicking POLG-SDs via knockin (KI; CRISPR/ Prime editing-based or commercial) to obtain an iPSC line carrying the most prevalent A467T mutation, and iPSCs with lentivirus-based shRNA-mediated knockdown of POLG. Reintroduction of human disease-associated POLG will shed light on the cellular effect and pathomechanisms of different disease-associated mutations. We will assay mitochondrial and neuronal function, aiming to establish a well-characterized human neuronal cell model of POLG-SDs. [...]
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